Hydrogen-rich Saline Protects Myocardium Against ischemia/reperfusion Injury in Rats

Exp Biol Med (Maywood). 2009 Oct;234(10):1212-9. doi: 10.3181/0812-RM-349. Epub 2009 Jul 13.

Abstract

Protective effect of hydrogen (H(2)) gas on cardiac ischemia-reperfusion (I/R) injury has been demonstrated previously. This study was designed to test the hypothesis that hydrogen-rich saline (saline saturated with molecular hydrogen), which is easy to use, induces cardioprotection against ischemia (30 min) and reperfusion (24 h) injury in rats. Adult male Sprague-Dawley rats underwent 30-min occlusion of the left anterior descending (LAD) coronary artery and 24-h reperfusion. Intraperitoneal injection of hydrogen-rich saline before reperfusion significantly decreased plasma and myocardium malondialdehyde (MDA) concentration, decreased cardiac cell apoptosis, and myocardial 8-hydroxydeoxyguanosine (8-OHdG) in area at risk zones (AAR), suppressed the activity of caspase-3, and reduced infarct size. The heart function parameters including left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP), +(dP/dt)(max) and -(dP/dt)(max) were also significantly improved 24 h after reperfusion. It is concluded that hydrogen-rich saline is a novel, simple, safe, and effective method to attenuate myocardial I/R injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Animals
  • Apoptosis / drug effects
  • Blood Pressure / drug effects
  • Cardiotonic Agents / pharmacology*
  • Caspase 3 / metabolism
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / metabolism
  • Hydrogen / pharmacology*
  • Male
  • Malondialdehyde / metabolism
  • Myocardial Reperfusion Injury / physiopathology
  • Myocardial Reperfusion Injury / prevention & control*
  • Myocardium / metabolism*
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Chloride / pharmacology*
  • Ventricular Dysfunction, Left / drug therapy

Substances

  • Cardiotonic Agents
  • Sodium Chloride
  • Malondialdehyde
  • Hydrogen
  • 8-Hydroxy-2'-Deoxyguanosine
  • Caspase 3
  • Deoxyguanosine