L-PGDS (betatrace protein) inhibits astrocyte proliferation and mitochondrial ATP production in vitro

J Mol Neurosci. 2009 Nov;39(3):366-71. doi: 10.1007/s12031-009-9214-7. Epub 2009 Jul 14.


L-PGDS is the most abundant protein present in the cerebrospinal fluid (CSF). Although CSF was believed to be homogenous in content, a previous study has showed that a marked concentration gradient of L-PGDS exists between the spinal CSF and the CSF in the subarachnoid space of patients with optic nerve disease (papilledema and normal-tension glaucoma). Astrocytes play a critical role in maintaining the integrity of axon function in the central nervous system and specifically in the optic nerve, and we therefore investigated the biochemical effects of L-PGDS on the proliferation of astrocytes and on the production of adenosine triphosphate (ATP) by astrocyte mitochondria. We found an inhibitory effect of L-PGDS on both proliferation of astrocytes and production of astrocyte ATP. The concentrations that inhibited astrocyte proliferation and ATP production were in the range measured in patients with idiopathic intracranial hypertension and in patients with normal-tension glaucoma. As the CSF is in contact with axons and mitochondria of the optic nerve (Bristow et al. Archives of Ophthalmology, 120, 791-796, 2002), we postulate that a change in the concentration of CSF protein such as L-PGDS could exercise a harmful effect on these structures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Astrocytes / drug effects
  • Astrocytes / metabolism*
  • Axons / metabolism
  • Axons / pathology
  • Axons / ultrastructure
  • Cell Line
  • Cell Proliferation / drug effects
  • Energy Metabolism / drug effects
  • Energy Metabolism / physiology
  • Glaucoma / cerebrospinal fluid
  • Glaucoma / physiopathology
  • Humans
  • Intramolecular Oxidoreductases / cerebrospinal fluid*
  • Intramolecular Oxidoreductases / pharmacology
  • Lipocalins / cerebrospinal fluid*
  • Lipocalins / pharmacology
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Optic Nerve / cytology
  • Optic Nerve / metabolism*
  • Optic Nerve / physiopathology
  • Optic Nerve Diseases / cerebrospinal fluid*
  • Optic Nerve Diseases / physiopathology
  • Pseudotumor Cerebri / cerebrospinal fluid
  • Pseudotumor Cerebri / physiopathology
  • Subarachnoid Space / cytology
  • Subarachnoid Space / metabolism*
  • Subarachnoid Space / physiopathology


  • Lipocalins
  • Adenosine Triphosphate
  • Intramolecular Oxidoreductases
  • prostaglandin R2 D-isomerase