Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Oct;138(4):629-37.
doi: 10.1530/REP-09-0144. Epub 2009 Jul 14.

Cumulus Gene Expression as a Predictor of Human Oocyte Fertilisation, Embryo Development and Competence to Establish a Pregnancy

Affiliations

Cumulus Gene Expression as a Predictor of Human Oocyte Fertilisation, Embryo Development and Competence to Establish a Pregnancy

R A Anderson et al. Reproduction. .

Abstract

The close relationship between cumulus cell function and oocyte developmental competence indicates that analysis of cumulus gene expression is a potential non-invasive method to aid embryo selection and IVF outcome. Cumulus was isolated from 674 oocytes from 75 women undergoing ICSI and gene expression analysed by quantitative RT-PCR. Cumulus expression of cyclooxygenase 2 (PTGS2) was higher with mature oocytes, whereas brain-derived neurotrophic factor (BDNF) was lower when fertilisation was normal. Expression levels of gremlin (GREM1) and BDNF were weak positive and negative predictors of embryo quality respectively. Ranking of GREM1 expression within cohorts of oocytes showed that oocytes associated with the highest GREM1 expression were more likely to be transferred or cryopreserved than discarded (49 vs 33%, P<0.02), although the clinical pregnancy rate was not significantly different. This study demonstrates both the feasibility and difficulties of this method of analysis in the largest such group studied thus far. Novel relationships between BDNF expression and fertilisation were identified, and the potential value of GREM1 expression as a marker of embryo quality supports the further assessment of GREM1 analysis in the context of embryo selection.

Similar articles

See all similar articles

Cited by 35 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback