Vesicular stomatitis virus as a novel cancer vaccine vector to prime antitumor immunity amenable to rapid boosting with adenovirus

Mol Ther. 2009 Oct;17(10):1814-21. doi: 10.1038/mt.2009.154. Epub 2009 Jul 14.


Vesicular stomatitis virus (VSV) has proven to be an effective vaccine vector for immunization against viral infection, but its potential to induce an immune response to a self-tumor antigen has not been investigated. We constructed a recombinant VSV expressing human dopachrome tautomerase (hDCT) and evaluated its immunogenicity in a murine melanoma model. Intranasal delivery of VSV-hDCT activated both CD4(+) and CD8(+) DCT-specific T-cell responses. The magnitude of these responses could be significantly increased by booster immunization with recombinant adenovirus (Ad)-hDCT, which led to enhanced efficacy against B16-F10 melanoma in both prophylactic and therapeutic settings. Notably, the interval of VSV/Ad heterologous vaccination could be shortened to as few as 4 days, making it a potential regimen to rapidly expand antigen-specific effector cells. Furthermore, VSV-hDCT could increase DCT-specific T-cell responses primed by Ad-hDCT, suggesting VSV is efficient for both priming and boosting of the immune response against a self-tumor antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • CD8-Positive T-Lymphocytes / immunology
  • Cancer Vaccines / genetics
  • Cancer Vaccines / immunology*
  • Cancer Vaccines / therapeutic use
  • Cell Line, Tumor
  • Female
  • Genetic Vectors / genetics*
  • Humans
  • Immunization, Secondary
  • Intramolecular Oxidoreductases / genetics
  • Intramolecular Oxidoreductases / immunology*
  • Lung Neoplasms / complications
  • Lung Neoplasms / immunology
  • Lung Neoplasms / therapy
  • Melanoma / immunology*
  • Melanoma / therapy
  • Mice
  • Mice, Inbred C57BL
  • T-Lymphocytes / immunology
  • Vesiculovirus / genetics*


  • Cancer Vaccines
  • Intramolecular Oxidoreductases
  • dopachrome isomerase