Real-time quantitative reverse transcription-PCR assays specifically detecting bluetongue virus serotypes 1, 6, and 8

J Clin Microbiol. 2009 Sep;47(9):2992-4. doi: 10.1128/JCM.00599-09. Epub 2009 Jul 15.

Abstract

Bluetongue virus (BTV) is a major pathogen of ruminants. Especially serotypes 1, 6, and 8 are of concern to veterinary authorities in central Europe. This article describes highly sensitive real-time reverse transcription-PCR assays directed to BTV genome segment 2 for specific detection of BTV-1, -6, or -8 in animal samples.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bluetongue / diagnosis*
  • Bluetongue / virology*
  • Bluetongue virus / classification*
  • Bluetongue virus / isolation & purification*
  • DNA Primers / genetics
  • Europe
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Ruminants
  • Sensitivity and Specificity

Substances

  • DNA Primers