Lutaonella thermophila gen. nov., sp. nov., a moderately thermophilic member of the family Flavobacteriaceae isolated from a coastal hot spring

Int J Syst Evol Microbiol. 2009 Aug;59(Pt 8):2069-73. doi: 10.1099/ijs.0.005256-0. Epub 2009 Jul 15.


A yellow-pigmented, Gram-staining-negative, aerobic, non-motile, moderately thermophilic, rod-shaped bacterium, CC-MHSW-2T, was isolated from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan. The 16S rRNA gene sequence analysis demonstrated that it shared <93.2% sequence similarity with Aquimarina species. The organism was unable to produce acid from carbohydrates, but it could utilize a number of organic acids and amino acids. Menaquinone 6 (MK-6) was the major respiratory quinone and iso-C15:0, iso-C17:0 3-OH and summed feature 3 (comprising C16:1omega7c and/or C16:1omega6c) were the predominant fatty acids. This fatty acid profile distinguished the isolate from members of the genera Aquimarina, Tamlana, Zhouia, Leeuwenhoekiella and Cellulophaga. The DNA G+C content of strain CC-MHSW-2T was 39.7+/-1 mol%. On the basis of the 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain CC-MHSW-2T represents a novel genus and species in the family Flavobacteriaceae, for which the name Lutaonella thermophila gen. nov., sp. nov. is proposed. The type strain is CC-MHSW-2T (=KCTC 22538T=JCM 15069T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Amino Acids / metabolism
  • Bacterial Typing Techniques
  • Bacteroidetes / classification*
  • Bacteroidetes / genetics
  • Bacteroidetes / isolation & purification*
  • Bacteroidetes / physiology
  • Base Composition
  • Carbohydrate Metabolism
  • Carboxylic Acids / metabolism
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Hot Springs / microbiology*
  • Locomotion
  • Molecular Sequence Data
  • Phylogeny
  • Pigments, Biological / biosynthesis
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Taiwan


  • Amino Acids
  • Carboxylic Acids
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Pigments, Biological
  • Quinones
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/EU287913