Assessment of phenolics-enriched extract and fractions of olive leaves and their antioxidant activities

Bioresour Technol. 2009 Dec;100(23):6107-13. doi: 10.1016/j.biortech.2009.06.059. Epub 2009 Jul 15.


Recent studies suggest that olive leaf is a significant source of bioactive phenolic compounds comparable to olive oil and fruits. Identifying appropriate extraction methods is thus an important step to increase the yield of such bioactive components from olive leaf, which is otherwise agricultural waste. The present study evaluates phenolic contents and compositions of olive leaf extracted by several solvent methods and to further establish their antioxidant activities using various radical scavenging systems. Total flavonoid and phenolic contents were significantly higher in the 80% ethanol extract, butanol, and ethylacetate fractions than hexane, chloroform and water fractions (p<0.05). Oleuropein was identified as a major phenolic compound with considerable contents in these major three fractions and the extract that correlated with their higher antioxidant and radical scavenging. These results indicate that olive leaf contains significant amounts of oleuropein and phenolics, important factors for antioxidant capacity, which can be substantially modified by different extraction methods.

MeSH terms

  • Agriculture / methods
  • Antioxidants / chemistry
  • Antioxidants / metabolism*
  • Chloroform / chemistry
  • Free Radical Scavengers
  • Hexanes / chemistry
  • Industrial Waste
  • Iridoids
  • Linoleic Acid / chemistry
  • Olea / metabolism*
  • Oxygen / chemistry
  • Peroxides / chemistry
  • Phenol
  • Phenols / chemistry*
  • Plant Extracts / pharmacology*
  • Pyrans / chemistry
  • Water / chemistry


  • Antioxidants
  • Free Radical Scavengers
  • Hexanes
  • Industrial Waste
  • Iridoids
  • Peroxides
  • Phenols
  • Plant Extracts
  • Pyrans
  • Water
  • oleuropein
  • Phenol
  • Chloroform
  • Linoleic Acid
  • Oxygen