[Expression study of DNA translesion synthesis genes in human primary glioma]

Zhonghua Yi Xue Za Zhi. 2009 May 19;89(19):1309-12.
[Article in Chinese]


Objective: To detect the expressions of Rev1, Pol zeta, Pol eta, Pol iota and Pol kappa mRNA in human primary glioma tissues, and to analyze the relationship between the expression and the pathological grade.

Methods: We applied SYBR Green real-time PCR to investigate the mRNA expressions of the 6 genes in 85 primary glioma tissues and 14 normal brain tissues.

Results: The expressions of 4 genes including Rev3, Rev7, Pol eta and Pol iota were significantly (P < 0.05) higher in glioma tissues in grade II, III and IV as compared to normal brain tissues. The expressions of Rev1 and Pol kappa were significantly (P < 0.05) higher in grade IV glioma compared to the normal brain tissue. The positive correlation existed (P < 0.01) between the expression of all genes mRNA and the pathologic grade of the human glioma, except for Rev7.

Conclusion: The up-regulation of DNA translesion synthesis genes, including Rev1, Rev3, Rev7, Pol eta, Pol iota and Pol kappa, may be associated with pathogenesis of glioma.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / pathology
  • Child
  • DNA Damage
  • DNA Repair / genetics*
  • DNA-Binding Proteins / genetics
  • DNA-Directed DNA Polymerase / genetics
  • Female
  • Gene Expression
  • Glioma / genetics*
  • Glioma / pathology
  • Humans
  • Mad2 Proteins
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Nuclear Proteins / genetics
  • Nucleotidyltransferases / genetics
  • Proteins / genetics
  • RNA, Messenger / genetics
  • Young Adult


  • DNA-Binding Proteins
  • MAD2L2 protein, human
  • Mad2 Proteins
  • Nuclear Proteins
  • Proteins
  • RNA, Messenger
  • Nucleotidyltransferases
  • REV1 protein, human
  • DNA-Directed DNA Polymerase
  • REV3L protein, human