Cytogenetic analysis of Populus trichocarpa--ribosomal DNA, telomere repeat sequence, and marker-selected BACs

Cytogenet Genome Res. 2009;125(1):74-80. doi: 10.1159/000218749. Epub 2009 Jul 14.

Abstract

The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics
  • Chromosomes, Artificial, Bacterial / genetics
  • Chromosomes, Plant / genetics
  • Cytogenetic Analysis
  • DNA, Plant / genetics*
  • DNA, Ribosomal / genetics*
  • Genetic Markers
  • In Situ Hybridization, Fluorescence
  • Populus / classification
  • Populus / genetics*
  • Repetitive Sequences, Nucleic Acid
  • Species Specificity
  • Telomere / genetics

Substances

  • DNA, Plant
  • DNA, Ribosomal
  • Genetic Markers