[Identification of mycobacteria by high-performance liquid chromatography]

Zh Mikrobiol Epidemiol Immunobiol. 2009 May-Jun:(3):64-6.
[Article in Russian]

Abstract

Aim: To compare results of mycobacteria identification by bacteriologic methods as well as by high-performance liquid chromatography (HPLC).

Materials and methods: Two hundred and eighty strains of mycobacteria isolated from respiratory specimens and identified by bacteriologic methods and HPLC were studied.

Results: It was established that results of HLPC use were highly correlated with results of microbiologic methods of mycobacteria identification: for identification of M. tuberculosis complex the correlation was 97.0%, for nontuberculous (NTM) slowly growing mycobacteria--95.3%, for quickly growing NTM--96.2% (overall--96.1%). Results of identification of mycobacteria by HPLC were ready in significantly shorter time-frame (during 24 hours).

Conclusion: HLPC method could be recommended for identification of mycobacteria in bacteriologic reference laboratories.

Publication types

  • English Abstract

MeSH terms

  • Chromatography, High Pressure Liquid*
  • Diagnosis, Differential
  • Humans
  • Mycobacterium / classification
  • Mycobacterium / isolation & purification*
  • Mycobacterium Infections / diagnosis*
  • Mycobacterium Infections / microbiology
  • Sensitivity and Specificity