Abstract
Amphotericin B (AmB)/poly(lactic acid)-b-poly(ethylene glycol) (PLA-b-PEG) nanoparticles coated with polysorbate 80 (Tween-80) were prepared by nanoprecipitation for transport across the blood-brain barrier (BBB). The effects of Tween-80 on the size and distribution, entrapment efficiency and release behavior of AmB/PLA-b-PEG nanoparticles were investigated. Furthermore, the brain targeting and curative effect of coated nanoparticles were also investigated. The entrapment efficiency was significantly enhanced when nanoparticles were coated with Tween-80. The prepared nanoparticles were spherical with homogeneous distribution. Drug concentration in mice brain was greatly enhanced, which indicated that the coated nanoparticles could get across the BBB. Meanwhile, the AmB/PLA-b-PEG nanoparticles are able to reduce the toxicity of AmB to liver, kidney and blood system with improved therapeutic effect.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amphotericin B / administration & dosage*
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Amphotericin B / adverse effects
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Amphotericin B / pharmacokinetics
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Amphotericin B / therapeutic use*
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Animals
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Anti-Infective Agents / administration & dosage*
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Anti-Infective Agents / adverse effects
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Anti-Infective Agents / pharmacokinetics
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Anti-Infective Agents / therapeutic use*
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Blood-Brain Barrier / metabolism
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Brain / drug effects
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Brain / metabolism
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Cryptococcus neoformans / drug effects
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Drug Carriers / chemistry
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Hematologic Tests
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Kidney / drug effects
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Kidney / metabolism
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Lactates / chemistry*
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Liver / drug effects
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Liver / metabolism
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Meningitis, Cryptococcal / drug therapy
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Mice
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Mice, Inbred BALB C
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Nanoparticles / chemistry*
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Nanoparticles / ultrastructure
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Polyethylene Glycols / chemistry*
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Polysorbates / chemistry*
Substances
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Anti-Infective Agents
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Drug Carriers
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Lactates
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Polysorbates
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poly(lactic acid-ethylene glycol)
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Polyethylene Glycols
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Amphotericin B