Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2009 Jul;3(4):171-6.
doi: 10.1111/j.1750-2659.2009.00086.x.

Performance of Influenza Rapid Point-Of-Care Tests in the Detection of Swine Lineage A(H1N1) Influenza Viruses

Affiliations
Free PMC article
Comparative Study

Performance of Influenza Rapid Point-Of-Care Tests in the Detection of Swine Lineage A(H1N1) Influenza Viruses

Aeron C Hurt et al. Influenza Other Respir Viruses. .
Free PMC article

Abstract

Background: In April 2009, an A(H1N1) influenza virus of swine lineage was detected in humans in the USA, and in just over a month has infected over 10,000 people in more than 40 countries.

Objectives: To determine the performance of the Binax Now, BD Directigen EZ, and the Quidel QuickVue influenza rapid point-of-care (POC) tests for the detection of the recently emerged swine lineage A(H1N1) virus.

Methods: Swine lineage A(H1N1) and human seasonal influenza strains were cultured and then diluted to specific infectivity titres. Viral dilutions were assayed by the rapid POC tests and by real-time RT-PCR.

Results: All three of the rapid POC tests successfully detected the swine lineage A(H1N1) viruses at levels between 10(3) and 10(5) TCID(50)/ml (tissue culture infectious dose(50)), with the BD Directigen test demonstrating marginally greater sensitivity than the other two tests. Viral infectivity and RNA load data for viruses at the detection limit of the rapid test kits, suggested that both the Quidel and the Binax tests were less sensitive for the detection of swine lineage A(H1N1) viruses than for human seasonal strains. In comparison the BD Directigen demonstrated similar sensitivity when detecting swine lineage A(H1N1) and human seasonal viruses.

Conclusions: The three rapid POC tests all detected the emergent swine lineage A(H1N1) virus when it was present at high virus concentrations. Early diagnosis of infection can assist in the rapid treatment. However the tests are significantly less sensitive than PCR assays and as such, negative results should be verified by a laboratory test.

Figures

Figure 1
Figure 1
Comparison of the sensitivity of rapid point‐of‐care (POC) tests with viral RNA levels in swine lineage A(H1N1) virus‐positive clinical specimens. Mean cycle threshold (Ct) values ± standard deviations were derived from the Ct values determined by real‐time RT‐PCR for either the three swine lineage viruses or the three human seasonal strains at the detection limit for the respective rapid POC test. Circles indicate the Ct values (derived from the same influenza A matrix real‐time RT‐PCR assay as used for the other experiment) for 10 different clinical specimens that were swine lineage A(H1N1) virus‐positive.

Comment in

Similar articles

See all similar articles

Cited by 47 articles

See all "Cited by" articles

References

    1. Shope RE. Swine influenza: I. Experimental transmission and pathology. J Exp Med 1931; 54:349–359. - PMC - PubMed
    1. Smith TF, Burgert EO Jr, Dowdle WR et al. Isolation of swine influenza virus from autopsy lung tissue of man. N Engl J Med 1976; 294:708–710. - PubMed
    1. Komadina N, Roque V, Thawatsupha P et al. Genetic analysis of two influenza A (H1) swine viruses isolated from humans in Thailand and the Philippines. Virus Genes 2007; 35:161–165. - PubMed
    1. Myers KP, Olsen CW, Gray GC. Cases of swine influenza in humans: a review of the literature. Clin Infect Dis 2007; 44:1084–1088. - PMC - PubMed
    1. Shinde V, Bridges CB, Uyeki TM et al. Triple‐reassortant swine influenza A (H1) in humans in the United States, 2005–2009. N Engl J Med 2009; Available at: http://www.ncbi.nlm.nih.gov/pubmed/19423871. (Accessed 21 May 2009). - PubMed

Publication types

MeSH terms

Feedback