The Integration-Excision System of the Conjugative Transposon Tn 1545 Is Structurally and Functionally Related to Those of Lambdoid Phages

Mol Microbiol. 1990 Sep;4(9):1513-21. doi: 10.1111/j.1365-2958.1990.tb02062.x.

Abstract

Excision of Tn1545 and related conjugative transposons of Gram-positive bacteria occurs by reciprocal site-specific recombination between non-homologous regions of the transposon-target junctions. Excisive recombination requires two transposon-encoded proteins designated Xis-Tn and Int-Tn. We have shown that, following excision, Tn1545 is a circular structure with ends separated by either of the two hexanucleotides that were present at the transposon-target junctions. Using a trans-complementation assay, we have demonstrated that Int-Tn is able to catalyse in vivo integration of a circular intermediate of Tn1545 defective for integration and excision. comparison of integration sites suggests that limited sequence homology at the vicinity of the recombining sites is required for integration of the element. These data support the hypothesis that the integration/excision systems of conjugative transposons from Gram-positive cocci and of lambdoid phages from Gram-negative bacilli have evolved from a common ancestor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage lambda / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Conjugation, Genetic*
  • DNA Transposable Elements*
  • DNA, Bacterial / genetics
  • DNA, Circular / genetics
  • Escherichia coli / genetics*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Recombination, Genetic
  • Sequence Homology, Nucleic Acid

Substances

  • DNA Transposable Elements
  • DNA, Bacterial
  • DNA, Circular