Release and spread of immunoreactive neurokinin A in the cat spinal cord in a model of acute arthritis

Brain Res. 1990 Nov 19;533(2):292-9. doi: 10.1016/0006-8993(90)91352-h.

Abstract

Antibody microprobes were used to study the release of immunoreactive neurokinin A into the spinal cord of anaesthetised cats during and following injection of a knee joint with kaolin and carrageenan. A basal level of immunoreactive neurokinin A was detected prior to any noxious stimuli. Innocuous mechanical joint stimuli (flexion or pressure) did not alter this basal level of release. However, on injection of kaolin and carrageenan into a knee joint, evidence of release into the ipsilateral spinal cord was immediately observed. Initially, immunoreactive neurokinin A was detected in 2 regions: one at the dorsal surface of the spinal cord and the other centred on the superficial dorsal horn. Within 1 h of joint injection, however, immunoreactive neurokinin A was detected throughout the dorsal horn and the adjacent white matter. The extensive spread and persistence of immunoreactive neurokinin A in the spinal cord may underlie some of the prolonged excitability changes evoked by brief noxious stimuli and peripheral inflammation reported by other laboratories.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Arthritis / chemically induced
  • Arthritis / metabolism*
  • Autoradiography
  • Carrageenan
  • Cats
  • Disease Models, Animal
  • Kaolin
  • Molecular Probes
  • Neurokinin A / metabolism*
  • Spinal Cord / metabolism*
  • Stress, Mechanical

Substances

  • Molecular Probes
  • Kaolin
  • Neurokinin A
  • Carrageenan