Isolation and purification of D-mannose from palm kernel

Carbohydr Res. 2009 Sep 8;344(13):1687-9. doi: 10.1016/j.carres.2009.06.018. Epub 2009 Jun 21.

Abstract

An economically viable procedure for the isolation and purification of d-mannose from palm kernel was developed in this research. The palm kernel was catalytically hydrolyzed with sulfuric acid at 100 degrees C and then fermented by mannan-degrading enzymes. The solution after fermentation underwent filtration in a silica gel column, desalination by ion-exchange resin, and crystallization in ethanol to produce pure d-mannose in a total yield of 48.4% (based on the weight of the palm kernel). Different enzymes were investigated, and the results indicated that endo-beta-mannanase was the best enzyme to promote the hydrolysis of the oligosaccharides isolated from the palm kernel. The pure d-mannose sample was characterized by FTIR, (1)H NMR, and (13)C NMR spectra.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Costs and Cost Analysis
  • Crystallization
  • Fermentation
  • Filtration
  • Glycoside Hydrolases / chemistry
  • Glycoside Hydrolases / metabolism
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Ion Exchange Resins / chemistry
  • Magnoliopsida / anatomy & histology*
  • Magnoliopsida / chemistry*
  • Mannose / chemistry
  • Mannose / isolation & purification*
  • Mannose / metabolism
  • Organic Chemicals / chemistry
  • Solvents / chemistry

Substances

  • Ion Exchange Resins
  • Organic Chemicals
  • Solvents
  • Glycoside Hydrolases
  • Mannose