G-TRACE: Rapid Gal4-based Cell Lineage Analysis in Drosophila

Nat Methods. 2009 Aug;6(8):603-5. doi: 10.1038/nmeth.1356. Epub 2009 Jul 26.

Abstract

We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Lineage*
  • Clone Cells
  • DNA Nucleotidyltransferases / genetics*
  • DNA-Binding Proteins / genetics*
  • Drosophila Proteins / genetics*
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Fluorometry
  • Genes, Reporter
  • Genetic Techniques*
  • Green Fluorescent Proteins / genetics
  • Open Reading Frames
  • Saccharomyces cerevisiae Proteins / genetics*
  • Transcription Factors / genetics*

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • GAL4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Green Fluorescent Proteins
  • DNA Nucleotidyltransferases
  • FLP recombinase