The twin spot generator for differential Drosophila lineage analysis

Nat Methods. 2009 Aug;6(8):600-2. doi: 10.1038/nmeth.1349. Epub 2009 Jul 26.


In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Lineage*
  • Clone Cells
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / genetics*
  • Fluorometry
  • Genomics / instrumentation
  • Genomics / methods*
  • Mitosis*
  • Mutation
  • Recombination, Genetic*