The transcription factor ITF-2A induces cell cycle arrest via p21(Cip1)

Biochem Biophys Res Commun. 2009 Oct 2;387(4):736-40. doi: 10.1016/j.bbrc.2009.07.102. Epub 2009 Jul 25.


Loss of heterozygosity (LOH) is a hallmark of cancer and the chromosomal regions 5q, 8p, 17p, and 18q are frequently affected by LOH in colorectal cancer. The ITF2 gene is located on chromosome 18q and we have recently demonstrated that expression of the basic helix-loop-helix (bHLH) transcription factor ITF-2B is affected by LOH. Apart from ITF-2B, the ITF2 gene also encodes the isoform ITF-2A that lacks amino-terminal sequences found in ITF-2B. Analysis of ITF-2A expression in micro-dissected colorectal tumor tissue revealed that ITF-2A expression is frequently lost in colorectal cancers, suggesting that loss of ITF-2A provides cancer cells with a growth advantage. Further studies demonstrated that re-expression of ITF-2A in colon cancer cell lines interferes with cell cycle progression. This data support the notion that the ITF2 gene on chromosome 18q is a tumor suppressor gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Cell Cycle / genetics
  • Cell Line, Tumor
  • Cell Proliferation
  • Chromosomes, Human, Pair 18 / genetics
  • Colorectal Neoplasms / genetics*
  • Cyclin-Dependent Kinase Inhibitor p21 / genetics*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation, Neoplastic*
  • Genes, Tumor Suppressor*
  • Humans
  • Loss of Heterozygosity*
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Transcription Factor 4
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic


  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • CDKN1A protein, human
  • Cyclin-Dependent Kinase Inhibitor p21
  • DNA-Binding Proteins
  • Protein Isoforms
  • TCF4 protein, human
  • Transcription Factor 4
  • Transcription Factors