A Radiorespirometric Method for Measuring Mineralization of [14C]-compounds in a 96-well Microplate Format

J Microbiol Methods. 2009 Oct;79(1):114-6. doi: 10.1016/j.mimet.2009.07.018. Epub 2009 Jul 25.

Abstract

A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Dioxide / metabolism*
  • Carbon Radioisotopes / metabolism*
  • Environmental Microbiology*
  • Environmental Monitoring
  • Staining and Labeling / methods*

Substances

  • Carbon Radioisotopes
  • Carbon Dioxide