An integrated microfluidic device for large-scale in situ click chemistry screening

Lab Chip. 2009 Aug 21;9(16):2281-5. doi: 10.1039/b907430a. Epub 2009 Jun 17.


An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetylene / chemistry
  • Animals
  • Azides / chemistry
  • Carbonic Anhydrase II / antagonists & inhibitors
  • Carbonic Anhydrase II / metabolism
  • Carbonic Anhydrase Inhibitors / analysis
  • Carbonic Anhydrase Inhibitors / pharmacology
  • Cattle
  • Microfluidic Analytical Techniques / instrumentation*
  • Solid Phase Extraction
  • Spectrometry, Mass, Electrospray Ionization
  • Systems Integration*


  • Azides
  • Carbonic Anhydrase Inhibitors
  • Carbonic Anhydrase II
  • Acetylene