Sequence-specific 1H, 13C, and 15N backbone assignment of the GTPase rRheb in its GDP-bound form

Biomol NMR Assign. 2007 Jul;1(1):45-7. doi: 10.1007/s12104-007-9013-4. Epub 2007 May 22.

Abstract

Rheb (Ras homologue enriched in brain) is a small GTPase that plays an important role in tuberous sclerosis. Here we present the backbone assignments of rRheb in its GDP-bound state. These assignments now provide a basis for the analysis of the interaction of rRheb with putative factors in order to elucidate the function of this GTPase and its role in the MAP kinase pathway of neuronal cells and in tuberous sclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Guanosine Diphosphate / chemistry
  • Guanosine Diphosphate / metabolism
  • Humans
  • In Vitro Techniques
  • MAP Kinase Signaling System
  • Molecular Structure
  • Monomeric GTP-Binding Proteins / chemistry*
  • Monomeric GTP-Binding Proteins / genetics
  • Monomeric GTP-Binding Proteins / metabolism
  • Neuropeptides / chemistry*
  • Neuropeptides / genetics
  • Neuropeptides / metabolism
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Binding
  • Ras Homolog Enriched in Brain Protein
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Tuberous Sclerosis / enzymology
  • Tuberous Sclerosis / genetics

Substances

  • Neuropeptides
  • RHEB protein, human
  • Ras Homolog Enriched in Brain Protein
  • Recombinant Proteins
  • Guanosine Diphosphate
  • Monomeric GTP-Binding Proteins