1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin I

Inés Castrillo; Jorge Alegre-Cebollada; Alvaro Martínez del Pozo; José G Gavilanes; Jorge Santoro; Marta Bruix

doi:10.1007/s12104-008-9127-3

1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin I

Biomol NMR Assign. 2009 Jun;3(1):5-7. doi: 10.1007/s12104-008-9127-3. Epub 2008 Nov 6.

Authors

Inés Castrillo¹, Jorge Alegre-Cebollada, Alvaro Martínez del Pozo, José G Gavilanes, Jorge Santoro, Marta Bruix

Affiliation

¹ Departamento de Espectroscopía y Estructura Molecular, Instituto de Química Física Rocasolano, CSIC, Serrano 119, 28006, Madrid, Spain.

PMID: 19636934
DOI: 10.1007/s12104-008-9127-3

Abstract

Sticholysin I is an actinoporin, a pore forming toxin, of 176 aminoacids produced by the sea anemone Stichodactyla heliantus. Isotopically labelled (13)C/(15)N recombinant protein was produced in E. coli. Here we report the complete NMR (15)N, (13)C and (1)H chemical shifts assignments of Stn I at pH 4.0 and 25 degrees C (BMRB No. 15927).

MeSH terms

Animals
Carbon Isotopes / chemistry
Magnetic Resonance Spectroscopy / methods*
Nitrogen Isotopes / chemistry
Organic Chemicals / chemistry
Pore Forming Cytotoxic Proteins / chemistry*
Protons
Sea Anemones / chemistry*

Substances

Carbon Isotopes
Nitrogen Isotopes
Organic Chemicals
Pore Forming Cytotoxic Proteins
Protons
stycholysin I