Metastatic effect of LY-6K gene in breast cancer cells

Int J Oncol. 2009 Sep;35(3):601-7. doi: 10.3892/ijo_00000371.


The over-expression of urokinase receptor in breast cancer cells results in increased tumor invasion, growth and metastasis. As LY-6K belongs to the Ly6/uPAR superfamily, we assessed the association of LY-6K with metastasis in breast cancer cell lines and how the LY-6K gene mediates cancer cell metastasis. The migration ability of cells in which expression of LY-6K gene is up- or down-regulated was investigated by transfecting LY-6K naked DNA and treating LY-6K siRNA. Assays of wound healing, migration and invasion were performed. The assays demonstrated that cell migration significantly increased when LY-6K gene is over-expressed or LY-6K protein is abnormally expressed in breast cancer cells. In addition, LY-6K caused cancer cell metastasis and it is linked to the Ras/ERK signaling pathway. Our results suggest that LY-6K meaningfully participates in breast cancer cell metastasis by influencing cell migration and invasion through the Ras/ERK pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Ly / genetics*
  • Biomarkers, Tumor / genetics
  • Blotting, Western
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology*
  • Cell Line, Tumor
  • Cell Movement / genetics*
  • Chromosomes, Artificial, Bacterial
  • Extracellular Signal-Regulated MAP Kinases / genetics
  • Female
  • Humans
  • Microscopy, Confocal
  • Neoplasm Invasiveness / genetics*
  • Neoplasm Invasiveness / pathology
  • Receptors, Urokinase Plasminogen Activator / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / genetics
  • Transfection


  • Antigens, Ly
  • Biomarkers, Tumor
  • Receptors, Urokinase Plasminogen Activator
  • Extracellular Signal-Regulated MAP Kinases