Immunoglobulin E cross-reactivity between lupine conglutins and peanut allergens in serum of lupine-allergic individuals

J Investig Allergol Clin Immunol. 2009;19(4):283-91.


Background: Lupine is used increasingly in food products. The development of lupine allergy in peanut-allergic patients is believed to occur as a result of cross-reactivity between lupine and peanut proteins.

Objective: To investigate the degree of immunoglobulin (Ig) E cross-reactivity between allergens in lupine and peanut.

Methods: We investigated IgE cross-reactivity between lupine alpha-, beta-, gamma-, and delta-conglutins and the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 using enzyme-linked immunosorbent assay with sera from patients with coexisting peanut and lupine allergy.

Results: Peanut proteins inhibited IgE binding towards alpha- conglutins, delta-conglutins, and, to a lesser degree, beta-conglutins, while no IgE cross-reaction with delta-conglutin was observed. Ara h 2 most potently inhibited IgE binding to lupine and delta-conglutins, while Ara h 1 most potently cross-reacted with beta-conglutin. Ara h 3 was apparently not involved in these mechanisms.

Conclusions: The present study reveals IgE cross-reactivity between the 2S albumins Ara h 2 and delta-conglutin, and the 7S vicilin-like Ara h 1 and beta-conglutin, which are possibly based on homologies between phylogenetically related proteins. Ara h 2 was the most potent inhibitor of IgE binding to lupine conglutins.

MeSH terms

  • 2S Albumins, Plant / immunology
  • Adolescent
  • Allergens / immunology
  • Antigens, Plant / immunology
  • Arachis / immunology*
  • Binding, Competitive
  • Child
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes
  • Female
  • Food Hypersensitivity / blood
  • Food Hypersensitivity / immunology*
  • Glycoproteins / immunology
  • Humans
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology*
  • Lupinus / immunology*
  • Male
  • Membrane Proteins
  • Peanut Hypersensitivity / blood
  • Peanut Hypersensitivity / immunology
  • Plant Proteins / immunology
  • Seed Storage Proteins / immunology
  • Sequence Homology, Amino Acid


  • 2S Albumins, Plant
  • Allergens
  • Antigens, Plant
  • Ara h 1 protein, Arachis hypogaea
  • Ara h 2 allergen, Arachis hypogaea
  • Epitopes
  • Glycoproteins
  • Membrane Proteins
  • Plant Proteins
  • Seed Storage Proteins
  • allergen Ara h3
  • conglutin protein, Lupinus angustifolius
  • Immunoglobulin E