Distribution of Insertion Sequence IS200 in Salmonella and Shigella

J Gen Microbiol. 1990 Dec;136(12):2555-60. doi: 10.1099/00221287-136-12-2555.

Abstract

Two DNA probes for the detection of insertion sequence IS200 by either Southern blotting or colony hybridization were constructed. One of the probes is a 300 bp EcoRI-HindIII fragment of IS200 cloned onto pBluescript KS(+); the other is a tail-to-tail dimer of the same fragment cloned onto pUC19. A survey of the presence of IS200 among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of Salmonella spp. examined contained one or more copies of insertion sequence IS200, with the exception of the subgenus I serovar S. agona in which IS200 is not found. Although insertion sequence IS200 was first considered a Salmonella-specific element, it also exists in many isolates of Shigella sonnei and Shigella flexneri, but not in Shigella dysenteriae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Probes
  • DNA Transposable Elements*
  • DNA, Bacterial / analysis*
  • Molecular Sequence Data
  • Restriction Mapping
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / isolation & purification
  • Shigella / genetics*
  • Shigella / isolation & purification

Substances

  • DNA Probes
  • DNA Transposable Elements
  • DNA, Bacterial