Transcriptional Activation of an Anti-Oxidant Mouse Pon2 Gene by Dexamethasone

BMB Rep. 2009 Jul 31;42(7):421-6. doi: 10.5483/bmbrep.2009.42.7.421.

Abstract

Glucocorticoids regulate multiple physiological processes such as metabolic homeostasis and immune response. Mouse Pon2 (mPon2) acts as an antioxidant to reduce cellular oxidative stress in cells. In this present study, we investigated the transcriptional regulation of mPon2 by glucocorticoids. In the presence of glucocorticoid analogue dexamethasone, the expression of mPon2 mRNA in cells was increased, whereas the expression was inhibited by a transcription inhibitor actinomycin D. Glucocorticoid receptors bound to the putative glucocorticoid response elements located between -593 bp and -575 bp of the mPon2 promoter. Transcriptional activity was completely blocked when the putative element was mutated. Taken together, these results suggest that the expression of the mPon2 gene is directly regulated by glucocorticoid-glucocorticoid receptor complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Aryldialkylphosphatase / genetics*
  • Aryldialkylphosphatase / metabolism
  • Dexamethasone / pharmacology*
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Glucocorticoids / pharmacology
  • Mice
  • NIH 3T3 Cells
  • Promoter Regions, Genetic / drug effects
  • Receptors, Glucocorticoid / physiology
  • Transcriptional Activation / drug effects

Substances

  • Antioxidants
  • Glucocorticoids
  • Receptors, Glucocorticoid
  • Dexamethasone
  • Aryldialkylphosphatase