Identification of an intermediate compartment involved in protein transport from endoplasmic reticulum to Golgi apparatus

Eur J Cell Biol. 1990 Dec;53(2):185-96.


We have studied the role of a previously described tubulovesicular compartment near the cis-Golgi apparatus in endoplasmic reticulum (ER)-to-Golgi protein transport by light and immunoelectron microscopy in Vero cells. The compartment is defined by a 53-kDa transmembrane protein designated p53. When transport of the vesicular stomatitis virus strain ts045 G protein was arrested at 39.5 degrees C, the G protein accumulated in the ER but had access to the p53 compartment. At 15 degrees C, the G protein was exported from the ER into the p53 compartment which formed a compact structure composed of vesicular and tubular profiles in close proximity to the Golgi. Upon raising the temperature to 32 degrees C, the G protein migrated through the Golgi apparatus while the p53 compartment resumed its normal structure again. These results establish the p53 compartment as the 15 degrees C intermediate of the ER-to-Golgi protein transport pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Endoplasmic Reticulum / metabolism*
  • Endoplasmic Reticulum / ultrastructure
  • Fluorescent Antibody Technique
  • Glycoproteins / metabolism*
  • Golgi Apparatus / metabolism*
  • Golgi Apparatus / ultrastructure
  • Membrane Glycoproteins*
  • Membrane Proteins / metabolism
  • Microscopy, Immunoelectron
  • Models, Biological
  • Organelles / metabolism
  • Organelles / ultrastructure
  • Temperature
  • Vero Cells
  • Vesicular stomatitis Indiana virus / metabolism*
  • Viral Envelope Proteins / metabolism*


  • G protein, vesicular stomatitis virus
  • Glycoproteins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Viral Envelope Proteins