[Effect of Flammulina Velutipes Polysaccharides on Production of Cytokines by Murine Immunocytes and Serum Levels of Cytokines in Tumor-Bearing Mice]

Zhong Yao Cai. 2009 Apr;32(4):561-3.
[Article in Chinese]

Abstract

Objective: To study the effect of Flammulina velutipes polysaccharides (FVP) on the production of tumor necrosis factor alpha (TNF-alpha), interferon-gamma (INF-gamma) and interleukin 2 (IL-2) by murine immunocytes.

Methods: The cell's metabolic activity was determined with methylthiazolyl tetrazolium (MTT) colorimetry assay and the amounts of TNF-alpha, INF-gamma and IL-2 were measured by ELISA.

Results: FVP (200, 100, 50 microg/mL) could promote the metabolic activity of murine splenocytes and peritoneal exudate cells (PEC) and increase the amounts of TNF-alpha, INF-gamma and IL-2 in the supernatants of splenocyte cultures, and the amount of TNF-alpha in PEC cultures, with the most marked increase on TNF-alpha level. FVP (100, 50, 25 mg/kg) could raise the serum levels of TNF-alpha and INF-gamma in S180 tumor-bearing mice.

Conclusion: FVP may regulate murine immune function through promoting the production of TNF-alpha, INF-gamma and IL-2.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / chemistry*
  • Animals
  • Cells, Cultured
  • Cytokines / blood
  • Cytokines / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / blood
  • Interleukin-2 / biosynthesis
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Peritoneum / cytology
  • Peritoneum / drug effects
  • Peritoneum / metabolism
  • Polysaccharides / administration & dosage
  • Polysaccharides / pharmacology*
  • Random Allocation
  • Sarcoma 180 / blood*
  • Sarcoma 180 / pathology
  • Spleen / cytology
  • Spleen / drug effects*
  • Spleen / metabolism
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Cytokines
  • Interleukin-2
  • Polysaccharides
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma