The expression of ENa(+)C and ASIC2 proteins in Pacinian corpuscles is differently regulated by TrkB and its ligands BDNF and NT-4

Neurosci Lett. 2009 Oct 2;463(2):114-8. doi: 10.1016/j.neulet.2009.07.073. Epub 2009 Jul 29.

Abstract

Pacinian corpuscles are innervated by large myelinated Aalpha-beta axons from the large- and intermediate-sized sensory neurons of dorsal root ganglia. These neurons express different members of the degenerin/epithelial Na(+) channel (DEG/ENa(+)C) superfamily of proteins with putative mechanosensory properties, whose expression is regulated by the TrkB-BDNF system. Thus, we hypothesized that BDNF and/or NT-4 signalling through activation of TrkB may regulate the expression of molecules supposed to be necessary for the mechanosensory function of Pacinian corpuscles. To test this hypothesis we analyzed the expression and distribution of ENa(+)C subunits and acid-sensing ion channel 2 (ASIC2) in Pacinian corpuscles from 25 days old mice deficient in TrkB, BDNF and NT-4. Pacinian corpuscles in these animals are normal in number, structure, and expression of several immunohistochemical markers. Using immunohistochemistry we observed that the beta-ENa(+)C and gamma-ENa(+)C subunits, but not the alpha-ENa(+)C subunit, were expressed in wild-type animals, and they were always found in the central axon. ASIC2 immunoreactivity was found in both the central axon and the inner core cells. The absence of TrkB or BDNF abolished expression of beta-ENa(+)C and ASIC2, whereas expression of gamma-ENa(+)C did not change. Expression of beta-ENa(+)C and gamma-ENa(+)C subunits in NT-4 deficient mice was found in the axons but also in the inner core cells whereas levels of expression of ASIC2 were increased in these animals. This study suggests that expression in Pacianian corpuscles of some potential mechanosensory proteins is regulated by BDNF, NT-4 and TrkB.

MeSH terms

  • Acid Sensing Ion Channels
  • Animals
  • Brain-Derived Neurotrophic Factor / genetics
  • Brain-Derived Neurotrophic Factor / physiology*
  • Degenerin Sodium Channels
  • Epithelial Sodium Channels / biosynthesis*
  • Immunohistochemistry
  • Ligands
  • Mechanotransduction, Cellular
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation
  • Nerve Growth Factors / genetics
  • Nerve Growth Factors / physiology*
  • Nerve Tissue Proteins / biosynthesis*
  • Pacinian Corpuscles / metabolism*
  • Protein Subunits / biosynthesis
  • Receptor, trkB / genetics
  • Receptor, trkB / physiology*
  • Sodium Channels / biosynthesis*

Substances

  • ASIC2 protein, mouse
  • Acid Sensing Ion Channels
  • Brain-Derived Neurotrophic Factor
  • Degenerin Sodium Channels
  • Epithelial Sodium Channels
  • Ligands
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • Protein Subunits
  • Sodium Channels
  • Receptor, trkB
  • neurotrophin 4