Sentinel lymph node accumulation of Lymphoseek and Tc-99m-sulfur colloid using a "2-day" protocol

Nucl Med Biol. 2009 Aug;36(6):687-92. doi: 10.1016/j.nucmedbio.2009.04.007.


Lymphoseek is a receptor-binding radiopharmaceutical specifically designed for sentinel lymph node (SLN) mapping. We conducted a clinical trial which measured the injection site clearance and sentinel lymph node accumulation after a single intradermal injection of Lymphoseek or unfiltered [(99m)Tc]sulfur colloid (TcSC) using a "2-day" protocol for SLN mapping of breast cancer. Eleven patients with breast cancer participated in this study. Five patients received an intradermal administration of 1.0 nmol of (99m)Tc-labeled Lymphoseek; SLN mapping was performed on four subjects within 19 to 27 h. Six subjects received an intradermal administration of TcSC; SLN mapping was performed on five subjects within 18 to 26 h. Lymphoseek exhibited a significantly (P<.001) faster injection site clearance than TcSC. The mean Lymphoseek clearance half-time was 2.18+/-1.09 h compared to 57.4+/-92.8 h for TcSC. The mean sentinel lymph node uptake of Lymphoseek (1.5+/-1.7%) and TcSC (3.5+/-3.1%) was statistically equivalent (P=.213). When an intradermal injection is employed, Lymphoseek demonstrated faster injection site clearance than unfiltered [(99m)Tc]sulfur colloid and persistent SLN accumulation for at least 24 h.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Aged
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Dextrans / administration & dosage
  • Dextrans / pharmacokinetics*
  • Female
  • Humans
  • Injections, Intradermal
  • Lymph Nodes / metabolism*
  • Mannans / administration & dosage
  • Mannans / pharmacokinetics*
  • Middle Aged
  • Organotechnetium Compounds / administration & dosage
  • Organotechnetium Compounds / pharmacokinetics*
  • Pentetic Acid / administration & dosage
  • Pentetic Acid / pharmacokinetics*
  • Radioactivity
  • Radiopharmaceuticals / administration & dosage
  • Radiopharmaceuticals / pharmacokinetics*
  • Sentinel Lymph Node Biopsy
  • Technetium Tc 99m Pentetate / analogs & derivatives
  • Technetium Tc 99m Sulfur Colloid / administration & dosage
  • Technetium Tc 99m Sulfur Colloid / pharmacokinetics*
  • Time Factors


  • Dextrans
  • Mannans
  • Organotechnetium Compounds
  • Radiopharmaceuticals
  • technetium-diethylenetriaminepentaacetic acid-mannosyl-dextran
  • Technetium Tc 99m Sulfur Colloid
  • Pentetic Acid
  • Technetium Tc 99m Pentetate