Psychrobacter piscatorii sp. nov., a psychrotolerant bacterium exhibiting high catalase activity isolated from an oxidative environment

Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):205-208. doi: 10.1099/ijs.0.010959-0. Epub 2009 Jul 31.


A Gram-negative, non-motile, psychrotolerant bacterium exhibiting high catalase activity, designated strain T-3-2(T), was isolated from a drain of a fish-processing plant. Its catalase activity was 12 000 U (mg protein)(-1), much higher than the activity of the other Psychrobacter strains tested. The strain grew at 0-30 degrees C and in the presence of 0-12 % NaCl. The predominant isoprenoid quinone was ubiquinone-8 (Q-8), and C(16 : 1)omega9c and C(18 : 1)omega9c were the predominant cellular fatty acids. The DNA G+C content of strain T-3-2(T) was 43.9 mol%. 16S rRNA gene sequence phylogeny suggested that strain T-3-2(T) is a member of the genus Psychrobacter, with the closest relatives being the type strains of Psychrobacter nivimaris (99.2 % similarity), P. aquimaris (98.7 %) and P. proteolyticus (98.5 %). DNA-DNA hybridization showed less than 65 % relatedness with these strains. A phylogenetic tree based on gyrB gene sequences was more reliable, with higher bootstrap values than the 16S rRNA gene sequence-based tree. The result also differentiated the isolate from previously reported Psychrobacter species. Owing to the significant differences in phenotypic and chemotaxonomic characteristics and the phylogenetic and DNA-DNA relatedness data, the isolate merits classification within a novel species, for which the name Psychrobacter piscatorii sp. nov. is proposed. The type strain is T-3-2(T) (=JCM 15603(T) =NCIMB 14510(T)).

MeSH terms

  • Animals
  • Bacterial Proteins / metabolism*
  • Base Composition
  • Catalase / metabolism*
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Fatty Acids / metabolism
  • Fishes
  • Food Handling
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Phylogeny
  • Psychrobacter / classification*
  • Psychrobacter / enzymology
  • Psychrobacter / genetics
  • Psychrobacter / isolation & purification*
  • RNA, Ribosomal, 16S / genetics
  • Sewage / microbiology*


  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Sewage
  • Catalase

Associated data

  • GENBANK/AB453700
  • GENBANK/AB490498