A potent metabotropic glutamate receptor agonist: electrophysiological actions of a conformationally restricted glutamate analogue in the rat spinal cord and Xenopus oocytes

Brain Res. 1990 Dec 24;537(1-2):311-4. doi: 10.1016/0006-8993(90)90375-l.

Abstract

The (2S,3S,4S) isomer of alpha-(carboxycyclopropyl)glycine (L-CCG-I), a conformationally restricted glutamate analogue, caused a marked depolarization of motoneurons in the isolated rat spinal cord, which was almost insensitive to CPP and CNQX. Depolarizing responses to L-CCG-I were markedly decreased by reducing the temperature of the bathing fluid. Similar results were obtained in the case of trans-ACPD, which is a metabotropic glutamate receptor agonist, but the depolarizing action of L-CCG-I was more potent than that of trans-ACPD. In Xenopus oocytes injected with poly(A)+ mRNA extracted from the rat brain, L-CCG-I induced significant oscillatory chloride currents, suggesting that L-CCG-I is a potent agonist for metabotropic-type glutamate receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids, Dicarboxylic / pharmacology*
  • Animals
  • Animals, Newborn / physiology
  • Brain / physiology
  • Cycloleucine / analogs & derivatives
  • Cycloleucine / pharmacology
  • Electrophysiology
  • Molecular Conformation
  • Oocytes / metabolism*
  • RNA, Messenger / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Receptors, Glutamate
  • Receptors, Neurotransmitter / drug effects*
  • Spinal Cord / metabolism*
  • Xenopus

Substances

  • Amino Acids, Dicarboxylic
  • RNA, Messenger
  • Receptors, Glutamate
  • Receptors, Neurotransmitter
  • Cycloleucine
  • 1-amino-1,3-dicarboxycyclopentane
  • (alpha-carboxycyclopropyl)glycine