Impact of alginate overproduction on attachment and biofilm architecture of a supermucoid Pseudomonas aeruginosa strain

Appl Environ Microbiol. 2009 Sep;75(18):6022-5. doi: 10.1128/AEM.01078-09. Epub 2009 Jul 31.

Abstract

The supermucoid Pseudomonas aeruginosa strain PDO300Deltaalg8(pBBR1MCS-5:alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates
  • Bacterial Adhesion*
  • Biofilms / growth & development*
  • Gene Deletion
  • Genes, Bacterial
  • Glucuronic Acid / biosynthesis
  • Glucuronic Acid / genetics
  • Hexuronic Acids
  • Polysaccharide-Lyases / metabolism
  • Pseudomonas aeruginosa / growth & development
  • Pseudomonas aeruginosa / metabolism
  • Pseudomonas aeruginosa / physiology*

Substances

  • Alginates
  • Hexuronic Acids
  • Glucuronic Acid
  • Polysaccharide-Lyases
  • poly(beta-D-mannuronate) lyase