Demonstration of antigenic variation among isolates of human cytomegalovirus using monoclonal antibodies and indirect ELISA

G S James; M J Cloonan

doi:10.1016/0166-0934(90)90072-n

Demonstration of antigenic variation among isolates of human cytomegalovirus using monoclonal antibodies and indirect ELISA

J Virol Methods. 1990 Dec;30(3):301-10. doi: 10.1016/0166-0934(90)90072-n.

Authors

G S James¹, M J Cloonan

Affiliation

¹ Department of Microbiology, Prince Henry Hospital, Little Bay, New South Wales, Australia.

PMID: 1964944
DOI: 10.1016/0166-0934(90)90072-n

Abstract

An indirect ELISA was developed for the detection of antigenic differences between isolates of human cytomegalovirus (HCMV) using a monoclonal antibody to an early (67 kDa) antigen. Antibody binding curves were analyzed using a microcomputer program (LISACRV) based on a nonlinear logistical model. The derivation of values for the average intrinsic association constant for seven isolates of HCMV and the prototype AD169 strain revealed significant differences between them. Because of the importance of HCMV as a pathogen, especially in immunosuppressed and AIDS patients, further investigation of the biological significance of differences between isolates of HCMV is clearly warranted.

MeSH terms

Antibodies, Monoclonal / immunology
Antigenic Variation*
Antigens, Viral / analysis*
Binding Sites
Cells, Cultured
Cytomegalovirus / immunology*
Electronic Data Processing
Enzyme-Linked Immunosorbent Assay
Humans

Substances

Antibodies, Monoclonal
Antigens, Viral