The effect of Bacillus subtilis mouth rinsing in patients with periodontitis

Eur J Clin Microbiol Infect Dis. 2009 Nov;28(11):1353-6. doi: 10.1007/s10096-009-0790-9. Epub 2009 Aug 1.


Bacillus subtilis is an effective probiotic product for prevention of enteric infections both in humans and animals. We hypothesized that a mouth rinse containing Bacillus subtilis should adhere to and colonize part of the oral bacteria on periodontal tissue. The rinsing ability of Extraction 300E (containing Bacillus subtilis: E-300) was compared with that of a mouth wash liquid , Neosteline Green (benzethonium chloride; NG) that is commonly used in Japan. Compared with NG rinsing, E-300 rinsing resulted in a marked change in the BANA-score. The mean BANA values (score +/- SD) over the course of the study from 0 to 30 days were 1.52 +/- 0.51 (p < or = 0.1) and 0.30 +/- 0.47 (p < or = 0.01) for E-300, and 1.56 +/- 0.51 and 0.93 +/- 0.68 for NG, respectively. Gingival Index also had improvement, while probing pocket depth and bleeding on probing showed small improvements. Mouth rinsing with E-300 significantly reduced periodontal pathogens compared with NG. These results suggest that Bacillus subtilis is an appropriate mouth rinse for patients with periodontitis.

MeSH terms

  • Administration, Oral
  • Adult
  • Animals
  • Bacillus subtilis / physiology*
  • Female
  • Humans
  • Japan
  • Male
  • Middle Aged
  • Periodontal Index
  • Periodontitis / pathology
  • Periodontitis / therapy*
  • Probiotics / administration & dosage
  • Probiotics / therapeutic use*