Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

Mol Cell Probes. 1990 Dec;4(6):435-43. doi: 10.1016/0890-8508(90)90002-h.


Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.

MeSH terms

  • Base Sequence
  • DNA Probes
  • DNA, Viral / genetics*
  • DNA-Directed DNA Polymerase / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • HIV / genetics*
  • Herpesvirus 4, Human / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction*
  • Thermus / enzymology*


  • DNA Probes
  • DNA, Viral
  • DNA-Directed DNA Polymerase