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. 2009 Aug 3;17(16):13354-64.
doi: 10.1364/oe.17.013354.

Deep Tissue Multiphoton Microscopy Using Longer Wavelength Excitation

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Deep Tissue Multiphoton Microscopy Using Longer Wavelength Excitation

Demirhan Kobat et al. Opt Express. .

Abstract

We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation to that achieved with 1280-nm excitation through in vivo and ex vivo TPM of fluorescently-labeled blood vessels in mouse brain. We achieved high contrast imaging of blood vessels at approximately twice the depth with 1280-nm excitation as with 775-nm excitation. An imaging depth of 1 mm can be achieved in in vivo imaging of adult mouse brains at 1280 nm with approximately 1-nJ pulse energy at the sample surface. Blood flow speed measurements at a depth of 900 mum are performed.

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