Luteimicrobium subarcticum gen. nov., sp. nov., a new member of the suborder Micrococcineae

Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):796-800. doi: 10.1099/ijs.0.014597-0. Epub 2009 Aug 5.

Abstract

A novel Gram-stain-positive bacterium, designated R19-04(T), was isolated from a soil sample from Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. Cells of strain R19-04( T) exhibited a rod-coccus cycle. The peptidoglycan type of the isolate was A4alpha; lysine was the diagnostic diamino acid. The predominant menaquinone was MK-8(H(2)) and the major fatty acid was anteiso-C(15 : 0). The polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 72.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain R19-04(T) represented a novel member of the suborder Micrococcineae. Its nearest phylogenetic neighbours were the members of the genus Oerskovia, with 16S rRNA gene sequence similarity of 97.0-97.1 %. However, strain R19-04(T) was clearly distinguishable from members of the genus Oerskovia and phylogenetically related genera in terms of chemotaxonomic characteristics. On the basis of the chemotaxonomic and phylogenetic characteristics, a novel genus and species are proposed, Luteimicrobium subarcticum gen. nov., sp. nov. The type strain of Luteimicrobium subarcticum is R19-04(T) (=NBRC 105647(T) =DSM 22413(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Actinomycetales / physiology
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Ribosomal / analysis
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Genes, rRNA
  • Japan
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Species Specificity

Substances

  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AB489904