Abstract
Autoradiographic imaging can localize 45Ca2+ selectively accumulated via the Ca2+, Mg2(+)-ATPase into endoplasmic reticulum stores in rat brain slices. 45Ca2+ accumulation is markedly stimulated by oxalate and displays a heterogeneous distribution which resembles the mRNA distribution for a sarcoendoplasmic reticulum Ca2+, Mg2(+)-ATPase. Inositol 1,4,5-triphosphate [I(1,4,5)P3] inhibits 45Ca2+ accumulation selectively into regions corresponding to those enriched in I(1,4,5)P3 receptor-binding sites and in Ca2+, -Mg2(+)-ATPase mRNA. Thus rat brain endoplasmic reticulum calcium stores are anatomically and functionally differentiated.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Autoradiography
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Brain / anatomy & histology
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Brain / metabolism*
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Ca(2+) Mg(2+)-ATPase / genetics
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Calcium / metabolism*
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Calcium Channels*
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Calcium Radioisotopes
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Calcium-Transporting ATPases / genetics
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Endoplasmic Reticulum / drug effects
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Endoplasmic Reticulum / metabolism*
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In Vitro Techniques
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Inositol 1,4,5-Trisphosphate / metabolism
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Inositol 1,4,5-Trisphosphate / pharmacology
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Inositol 1,4,5-Trisphosphate Receptors
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Male
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Oxalates / pharmacology
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Rats
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Rats, Inbred Strains
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Receptors, Cell Surface / metabolism
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Receptors, Cytoplasmic and Nuclear*
Substances
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Calcium Channels
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Calcium Radioisotopes
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Inositol 1,4,5-Trisphosphate Receptors
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Oxalates
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RNA, Messenger
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Receptors, Cell Surface
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Receptors, Cytoplasmic and Nuclear
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Inositol 1,4,5-Trisphosphate
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Ca(2+) Mg(2+)-ATPase
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Calcium-Transporting ATPases
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Calcium