A diet enriched in polyphenols and polyunsaturated fatty acids, LMN diet, induces neurogenesis in the subventricular zone and hippocampus of adult mouse brain

J Alzheimers Dis. 2009;18(4):849-65. doi: 10.3233/JAD-2009-1188.


At present it is widely accepted that there are at least two neurogenic sites in the adult mammalian brain: the subventricular zone (SVZ) of lateral ventricles and the subgranular zone (SGZ) of the hippocampus dentate gyrus. The adult proliferation rate declines with aging and is altered in several neurodegenerative pathologies including Alzheimer's disease. The aim of this work was to study whether a natural diet rich in polyphenols and polyunsaturated fatty acids (LMN diet) can modulate neurogenesis in adult mice and give insight into putative mechanisms. Results with BrdU and PCNA demonstrated that the LMN fed mice had more newly generated cells in the SVZ and SGZ, and those with DCX (undifferentiated neurons) and tyrosine hydroxylase, calretinin, and calbindin (differentiated neurons) immunostainings and western blots demonstrated a significant effect on neuronal populations, strongly supporting a positive role of the LMN diet on adult neurogenesis. In primary rat neuron cultures, the LMN cream dramatically protected against damage caused by both hydrogen peroxide and Abeta(1-42), demonstrating a potent antioxidant effect that could play a major role in the normal adult neurogenesis and, moreover, the LMN diet could have a significant effect combating the cognitive function decline during both aging and neurodegenerative diseases such as Alzheimer's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides / drug effects
  • Amyloid beta-Peptides / physiology
  • Animals
  • Blotting, Western
  • Cerebral Ventricles / cytology*
  • Cerebral Ventricles / drug effects
  • Dentate Gyrus / cytology*
  • Dentate Gyrus / drug effects
  • Dietary Fats, Unsaturated / pharmacology
  • Dietary Fiber / pharmacology*
  • Doublecortin Protein
  • Fatty Acids, Unsaturated / pharmacology*
  • Flavonoids / pharmacology*
  • Food, Fortified
  • Immunohistochemistry
  • Male
  • Mice
  • Neurodegenerative Diseases / diet therapy
  • Neurodegenerative Diseases / metabolism
  • Neurogenesis / drug effects*
  • Neurogenesis / physiology
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology
  • Peptide Fragments / drug effects
  • Peptide Fragments / physiology
  • Phenols / pharmacology*
  • Polyphenols


  • Amyloid beta-Peptides
  • Dcx protein, mouse
  • Dcx protein, rat
  • Dietary Fats, Unsaturated
  • Dietary Fiber
  • Doublecortin Protein
  • Fatty Acids, Unsaturated
  • Flavonoids
  • Peptide Fragments
  • Phenols
  • Polyphenols
  • amyloid beta-protein (1-42)