Purification and Characterization of a Tuliposide-Converting Enzyme From Bulbs of Tulipa Gesneriana

Biosci Biotechnol Biochem. 2009 Aug;73(8):1895-7. doi: 10.1271/bbb.90226. Epub 2009 Aug 7.

Abstract

An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral pH and moderate temperature. The enzyme preferentially acted on such glucose esters as 6-tuliposides, and to a lesser extent on p-nitrophenylacetate.

MeSH terms

  • Anti-Infective Agents / metabolism
  • Biocatalysis
  • Glycosides / metabolism*
  • Hydrogen-Ion Concentration
  • Plant Shoots / enzymology*
  • Plant Shoots / metabolism
  • Sodium Oxybate / analogs & derivatives*
  • Sodium Oxybate / metabolism
  • Temperature
  • Tulipa / enzymology*
  • Tulipa / metabolism

Substances

  • Anti-Infective Agents
  • Glycosides
  • 6-tuliposide A
  • Sodium Oxybate