Anti-inflammatory effect of leaves of Eriobotrya japonica correlating with attenuation of p38 MAPK, ERK, and NF-kappaB activation in mast cells

Toxicol In Vitro. 2009 Oct;23(7):1215-9. doi: 10.1016/j.tiv.2009.07.036. Epub 2009 Aug 7.

Abstract

Mast cell-mediated allergic inflammation is involved in many diseases such as asthma and sinusitis. Mast cells induce synthesis and production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 with immune regulatory properties. In the present study, we investigate the effect an unspecified aqueous extract from leaves of Eriobotrya japonica Lindl. (Rosaceae) (LEJL) on the expression of pro-inflammatory cytokines and its possible mechanisms of action in human mast cells (HMC-1). LEJL dose-dependently inhibited phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187 (PMACI)-induced gene expression and secretion of TNF-alpha, IL-6, and IL-8. LEJL attenuated PMACI-induced activation of nuclear factor (NF)-kappaB, and specifically blocked activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK) but not that of c-jun N-terminal kinase. The inhibitory effect of LEJL on the pro-inflammatory cytokines was likely NF-kappaB, p38 MAPK, and ERK dependent. Our in vitro studies provide evidence that LEJL might contribute to the treatment of mast cell-derived allergic inflammatory diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology*
  • Cell Line
  • Eriobotrya*
  • Extracellular Signal-Regulated MAP Kinases / metabolism*
  • Gene Expression Regulation / drug effects
  • Humans
  • Mast Cells / drug effects*
  • Mast Cells / enzymology
  • Mast Cells / immunology
  • NF-kappa B / metabolism*
  • Plant Extracts / pharmacology*
  • Plant Leaves
  • RNA, Messenger / metabolism
  • p38 Mitogen-Activated Protein Kinases / metabolism*

Substances

  • Anti-Inflammatory Agents
  • NF-kappa B
  • Plant Extracts
  • RNA, Messenger
  • Extracellular Signal-Regulated MAP Kinases
  • p38 Mitogen-Activated Protein Kinases