Anaerostipes butyraticus sp. nov., an anaerobic, butyrate-producing bacterium from Clostridium cluster XIVa isolated from broiler chicken caecal content, and emended description of the genus Anaerostipes

Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1108-1112. doi: 10.1099/ijs.0.015289-0. Epub 2009 Aug 7.


Four butyrate-producing isolates were obtained from the caecal content of a 4-week-old broiler chicken. The 16S rRNA gene sequences were determined and confirmed the close relatedness of the four isolates, which suggested that they were derived from a single bacterial clone. Phylogenetic analysis based on 16S rRNA gene sequences showed that its closest relatives were members of cluster XIVa of the Clostridium subphylum of Gram-positive bacteria and that the closest related type strain was Anaerostipes caccae L1-92(T) (94.5 % similarity). Similarity levels of 96-98 % with sequences from uncultured bacteria from human stool samples were observed. On the basis of morphological, biochemical and phylogenetic characteristics, this strain is assigned to a novel species in the genus Anaerostipes, for which the name Anaerostipes butyraticus sp. nov. is proposed. The type strain is 35-7(T) (=LMG 24724(T) =DSM 22094(T)). An emended description of the genus Anaerostipes is also provided.

MeSH terms

  • Anaerobiosis
  • Animals
  • Bacterial Typing Techniques
  • Belgium
  • Butyrates / metabolism*
  • Cecum / microbiology*
  • Chickens / microbiology*
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Genes, rRNA
  • Genotype
  • Gram-Positive Asporogenous Rods / classification*
  • Gram-Positive Asporogenous Rods / genetics
  • Gram-Positive Asporogenous Rods / isolation & purification*
  • Gram-Positive Asporogenous Rods / physiology
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Species Specificity


  • Butyrates
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/FJ947528