Bicaudal C, a Novel Regulator of Dvl Signaling Abutting RNA-processing Bodies, Controls Cilia Orientation and Leftward Flow

Development. 2009 Sep;136(17):3019-30. doi: 10.1242/dev.038174.

Abstract

Polycystic diseases and left-right (LR) axis malformations are frequently linked to cilia defects. Renal cysts also arise in mice and frogs lacking Bicaudal C (BicC), a conserved RNA-binding protein containing K-homology (KH) domains and a sterile alpha motif (SAM). However, a role for BicC in cilia function has not been demonstrated. Here, we report that targeted inactivation of BicC randomizes left-right (LR) asymmetry by disrupting the planar alignment of motile cilia required for cilia-driven fluid flow. Furthermore, depending on its SAM domain, BicC can uncouple Dvl2 signaling from the canonical Wnt pathway, which has been implicated in antagonizing planar cell polarity (PCP). The SAM domain concentrates BicC in cytoplasmic structures harboring RNA-processing bodies (P-bodies) and Dvl2. These results suggest a model whereby BicC links the orientation of cilia with PCP, possibly by regulating RNA silencing in P-bodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Animals
  • Body Patterning / physiology*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cell Polarity*
  • Cilia* / metabolism
  • Cilia* / ultrastructure
  • Dishevelled Proteins
  • Embryo, Mammalian / abnormalities
  • Embryo, Mammalian / anatomy & histology
  • Embryo, Mammalian / physiology
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nodal Protein / genetics
  • Nodal Protein / metabolism
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • RNA Interference
  • RNA-Binding Proteins
  • Signal Transduction / physiology*
  • Wnt Proteins / genetics
  • Wnt Proteins / metabolism
  • Xenopus Proteins
  • Xenopus laevis / anatomy & histology
  • Xenopus laevis / embryology
  • Xenopus laevis / genetics
  • Xenopus laevis / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • Bicc1 protein, mouse
  • Carrier Proteins
  • DVL1 protein, Xenopus
  • DVL2 protein, human
  • Dishevelled Proteins
  • Dvl2 protein, mouse
  • Nodal Protein
  • Nodal protein, mouse
  • Phosphoproteins
  • RNA-Binding Proteins
  • Wnt Proteins
  • Xenopus Proteins