Insights from genomic profiling of transcription factors

Nat Rev Genet. 2009 Sep;10(9):605-16. doi: 10.1038/nrg2636. Epub 2009 Aug 11.


A crucial question in the field of gene regulation is whether the location at which a transcription factor binds influences its effectiveness or the mechanism by which it regulates transcription. Comprehensive transcription factor binding maps are needed to address these issues, and genome-wide mapping is now possible thanks to the technological advances of ChIP-chip and ChIP-seq. This Review discusses how recent genomic profiling of transcription factors gives insight into how binding specificity is achieved and what features of chromatin influence the ability of transcription factors to interact with the genome. It also suggests future experiments that may further our understanding of the causes and consequences of transcription factor-genome interactions.

Publication types

  • Review

MeSH terms

  • Animals
  • Binding Sites
  • Chromosome Mapping / methods*
  • Consensus Sequence / genetics
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation / genetics*
  • Humans
  • Models, Biological
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*


  • Transcription Factors