Microfluidic devices for measuring gene network dynamics in single cells

Nat Rev Genet. 2009 Sep;10(9):628-38. doi: 10.1038/nrg2625. Epub 2009 Aug 11.

Abstract

The dynamics governing gene regulation have an important role in determining the phenotype of a cell or organism. From processing extracellular signals to generating internal rhythms, gene networks are central to many time-dependent cellular processes. Recent technological advances now make it possible to track the dynamics of gene networks in single cells under various environmental conditions using microfluidic 'lab-on-a-chip' devices, and researchers are using these new techniques to analyse cellular dynamics and discover regulatory mechanisms. These technologies are expected to yield novel insights and allow the construction of mathematical models that more accurately describe the complex dynamics of gene regulation.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Cells / cytology
  • Cells / metabolism*
  • Gene Expression Regulation
  • Gene Regulatory Networks / physiology*
  • Green Fluorescent Proteins / analysis
  • Humans
  • Kinetics
  • Microfluidic Analytical Techniques* / trends
  • Models, Biological

Substances

  • Green Fluorescent Proteins