Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl)aminomethane buffer between 390 and 880 nm

Appl Opt. 2009 Aug 10;48(23):4610-5. doi: 10.1364/ao.48.004610.

Abstract

The refractivity of wild-type bacteriorhodopsin (bR(WT)) suspended in tris(hydroxymethyl)aminomethane (TRIS) buffer has been measured in the spectral range of 390-840 nm by the method of angle of minimal deviation with the use of a hollow glass prism. The refractive indices of pure bR(WT) as well as of TRIS buffer have been determined from the concentration dependent refraction values. Sellmeier-type dispersion equations have been fitted for both the TRIS buffer and pure bR(WT).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriorhodopsins / chemistry*
  • Buffers
  • Dose-Response Relationship, Drug
  • Glass
  • Halobacterium / metabolism
  • Hydrogen-Ion Concentration
  • Models, Statistical
  • Optics and Photonics*
  • Pressure
  • Refractometry
  • Reproducibility of Results
  • Temperature
  • Tromethamine / chemistry*

Substances

  • Buffers
  • Tromethamine
  • Bacteriorhodopsins