Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells

Mol Biosyst. 2009 Sep;5(9):931-4. doi: 10.1039/b904228h. Epub 2009 Jul 2.

Abstract

Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry
  • Amino Acids / metabolism*
  • Amino Acyl-tRNA Synthetases / chemistry
  • Amino Acyl-tRNA Synthetases / genetics
  • Amino Acyl-tRNA Synthetases / metabolism*
  • Azides / metabolism
  • Cell Line
  • Cross-Linking Reagents / metabolism
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Genes, Bacterial
  • HeLa Cells
  • Humans
  • Models, Molecular
  • Mutation
  • Phenylalanine / analogs & derivatives
  • Phenylalanine / metabolism
  • Species Specificity
  • Transfection

Substances

  • Amino Acids
  • Azides
  • Cross-Linking Reagents
  • 4-azidophenylalanine
  • Phenylalanine
  • Amino Acyl-tRNA Synthetases