A self-adapting approach for the detection of bursts and network bursts in neuronal cultures

J Comput Neurosci. 2010 Aug;29(1-2):213-229. doi: 10.1007/s10827-009-0175-1. Epub 2009 Aug 8.

Abstract

Dissociated networks of neurons typically exhibit bursting behavior, whose features are strongly influenced by the age of the culture, by chemical/electrical stimulation or by environmental conditions. To help the experimenter in identifying the changes possibly induced by specific protocols, we developed a self-adapting method for detecting both bursts and network bursts from electrophysiological activity recorded by means of micro-electrode arrays. The algorithm is based on the computation of the logarithmic inter-spike interval histogram and automatically detects the best threshold to distinguish between inter- and intra-burst inter-spike intervals for each recording channel of the array. An analogous procedure is followed for the detection of network bursts, looking for sequences of closely spaced single-channel bursts. We tested our algorithm on recordings of spontaneous as well as chemically stimulated activity, comparing its performance to other methods available in the literature.

MeSH terms

  • Action Potentials / drug effects
  • Action Potentials / physiology*
  • Adaptation, Physiological / drug effects
  • Adaptation, Physiological / physiology*
  • Algorithms
  • Animals
  • Bicuculline / pharmacology
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Computer Simulation
  • Electrodes
  • Embryo, Mammalian
  • Excitatory Amino Acid Antagonists / pharmacology
  • GABA-A Receptor Antagonists / pharmacology
  • Nerve Net / drug effects
  • Nerve Net / physiology*
  • Neurons / drug effects
  • Neurons / physiology*
  • Probability
  • Rats
  • Time Factors
  • Valine / analogs & derivatives
  • Valine / pharmacology

Substances

  • Excitatory Amino Acid Antagonists
  • GABA-A Receptor Antagonists
  • 2-amino-5-phosphopentanoic acid
  • Valine
  • Bicuculline