Surface expression of CD-4 does not predict susceptibility to infection with HIV-1 in human monocyte hybridomas

J Clin Lab Immunol. 1990 Apr;31(4):151-6.


In contrast to T-cell lines, where CD-4 expression may predict susceptibility to HIV infection, in monocyte hybridomas, presence or absence of surface CD-4 does not appear to be the determining factor of susceptibility to HIV infection. One clone, 20, was documented to be CD-4 negative by surface immunofluorescence as well as by immunoprecipitation. Both CD-4+ and CD-4- human monocyte hybridomas, representative of peripheral blood monocytes were readily infected with HIV (strains IIIB and BR-1 and a variety of patient isolates) as assessed by p24 Ag secretion reverse transcriptase activity and in situ hybridization. Infection occurred in the absence of antibody to HIV suggesting a non Fc mediated process as had been previously described. These data suggest that alternative mechanisms, such as non-specific phagocytosis, may exist for entry of HIV into peripheral blood monocytes. Given these findings, treatment for AIDS, such as the use of soluble CD-4, may not be effective long term, as monocyte infection may still occur and serve as a reservoir for subsequent viral infection of T cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • CD4 Antigens / analysis*
  • CD4 Antigens / physiology
  • HIV-1 / physiology*
  • Humans
  • Hybridomas / immunology
  • Hybridomas / microbiology*
  • Interferon-gamma / pharmacology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / microbiology
  • Lymphocyte Activation
  • Lymphoma, Large B-Cell, Diffuse / pathology
  • Phagocytosis
  • Receptors, HIV
  • Tumor Cells, Cultured / immunology
  • Tumor Cells, Cultured / microbiology


  • CD4 Antigens
  • Receptors, HIV
  • Interferon-gamma